This 'hands-on' tool shows the effect of five essential separation parameters:

1. Type of chromatographic system
2. Column length
3. Column diameter
4. Total pressure drop
5. Temperature.

You can change the various parameters in the red boxes (the cells B8; B9; B10; B13 and A2 of the sheet "improved system") and the generator calculates and shows the new chromatogram. The generator has been prepared by combining real experiments in packed bed, monolith and halo systems with the well known separation formulas. Calculations therefore appear quite useful for a first use in optimisation.

For more detail on the chromatogram generator and how to use it, click this link to an interview with Jeroen Billen and Gert Desmet.

Click this illustration to download file.
This is an Excel file, downloading may take a few seconds.Jeroen Billen, Gert Desmet. 2008